DNA repair mechanisms and disease

Department                           Molecular Genetics

Principal investigator          Hannes Lans

E-mail address                      w.lans@erasmusmc.nl

Website                                  https://lanslab.eu/


DNA repair responses to anti-cancer chemotherapy

DNA damaging chemotherapeutic drugs are clinically widely applied to kill cancer cells. Within cells, an elaborate network of DNA repair mechanisms protects against this type of DNA damage, to safeguard genomic integrity and ensure that essential processes such as replication and transcription can continue. In this project, we investigate the function and activity of known and new DNA repair proteins and mechanisms that are needed to detect and remove chemotherapy-induced DNA damage. Knowledge of these mechanisms is essential to understand how cancer treatment can be improved.


  • Cell culture
  • DNA repair assays
  • Live cell confocal imaging
  • Western blot
  • CRISPR-Cas9
  • Immunoprecipitation

Further reading

Slyskova, J., et al. (2018). Nucleic Acids Research, 46, 9537-9549 DOI: 10.1093/nar/gky764.