Department Cell Biology
Principal investigator Gert Jansen
E-mail address g.jansen@erasmusmc.nl
Molecular mechanism of neuronal cell fate maintenance
Supervisor: Manon Vleeming, m.vleeming@erasmusmc.nl
To maintain their proper functioning, cells must maintain their fate. However the molecular mechanisms that maintain cell fate are not well understood. We study this process in the ASE salt sensing neurons of C. elegans. We recently identified a transcription factor that contributes to ASE fate maintenance. In this project we will study the function of the transcription factor using molecular genetics, imaging techniques and behavioral assays.
Techniques
- C. elegans culture
- Crosses
- Microinjections
- CRISPR/Cas9 genome editing
- Auxin induced protein depletion
- PCR
- Fluorescence microscopy
- Chemotaxis assays
- Touch assays
Further reading
Traets, J.J.H., et al. (2021). eLife, 10, e66955. DOI: 10.7554/eLife.66955.