Department Bionanoscience
Principal investigator Kristin Grussmayer
E-mail address k.s.grussmayer@tudelft.nl
Website www.tudelft.nl/grussmayerlab
Quantitative imaging of cytoskeletal crosstalk at the nanoscale
Supervisor: Moritz Engelhardt; in collaboration with Koenderink lab
Master project to establish new multicolor super-resolution imaging to unveil the nanoscopic cytoskeletal structures (actin, microtubules and intermediate filaments) of cells as well as their interaction (crosstalk) and relate it to their active mechanical behaviour. You will optimize multicolor imaging and focus on quantitative network analysis of multicolor super-resolved networks at the nanoscale or on extending imaging from 2D to 3D. At a later stage, we will investigate how different surface micropatterning influences cytoskeletal crosstalk. This is a joint Kavli Institute for Nanotechnology Delft funded project between the Koenderink Lab and the Grussmayer Lab @BN/AS.
Techniques
- Cell culture and labelling
- State-of-the-art super-resolution microscopy methods
- Advanced image analysis
- Visualization and presentation of experimental data
- Micropatterning of surfaces for controlled cell adhesion
Gentle super-resolution imaging for tissue
In collaboration with the Smith Lab at 3mE
Master project super-resolution imaging for optically “complex” biomedical samples by combining Super-resolution optical fluctuation imaging on a new light sheet microscope (SOLEIL) with adaptive optics. Joint TU Delft Bioengineering Institute funded project between the Smith Lab @DCSC/3ME and the Grussmayer Lab @BN/AS.
Techniques
- Cell culture and labelling
- State-of-the-art super-resolution microscopy methods
- Adaptive optics
- Data simulation
- Adaptive optics algorithm
- Microscopy hardware for simultaneous 3D imaging