Department Imaging Physics
Principal investigator Bernd Rieger
E-mail address b.rieger@tudelft.nl
Website https://home.imphys.tudelft.nl/~brieger/
Magnetic control of the brightness of fluorescent proteins
Supervisor: Bernd Rieger, b.rieger@tudelft.nl
That the fluorescence of molecules can be influenced by e.g. photo conversion from UV light is long known, but that magnetic field also allow this is new. We want to study a group of fluorescent proteins developed by Andrew York and Maria Ingaramo at Calico Life Sciences for the use in super-resolution microscopy for single molecule localization microscopy (SMLM) and Structure Illumination Microscopy (SIM).
Techniques
- Fluorescence Microscopy
Further reading
https://andrewgyork.github.io/gfp_magnetofluorescence/
Nuclear lamin network distribution analysis
Supervisor: Bernd Rieger, b.rieger@tudelft.nl
Together with Hylkje Geertsma we image nuclear lamin proteins in 3D with the help of DNA-PAINT. The network of the proteins shows tunnels in the nucleus. We want to analyse the distribution of the proteins on the surface of the nuclear envelope. We hypothesis that the density of the proteins must be different dependent on the local curvature.
Techniques
- Image Analysis
- Super resolution single molecule localization microscopy